DNA coding for a human vasoconstrictive peptide and use thereof

U.S. Patent Number: 5756344

Abstract: Disclosed are (1) a DNA containing a cDNA segment coding for human endothelin-2 (SEQ ID NO:1), (2) a precursor of human endothelin-2 (SEQ ID NO:2), (3) a transformant carrying a DNA containing a cDNA segment coding for human endothelin-2, and (4) a method for preparing mature human endothelin-2 which comprises culturing the transformant described in (3), accumulating a protein in a culture medium, and collecting the same, whereby human endothelin-2 and the precursors thereof can be produced in large amounts.

Inventors: Onda; Haruo (Tsukuba Ibaraki, JP), Ohkubo; Shoichi (Tsukuba Ibaraki, JP), Kosaka; Takuo (Tsukuba Ibaraki, JP)

Assignee: Takeda Chemical Industries, Ltd. (

Application Number: 08/673,269

Issued: 1998-05-26

Expired: 2006-05-26

Classes: 435/252.3 ; 435/252.33; 435/320.1; 435/325; 435/69.4; 530/315; 530/324; 536/23.51

Field of search: 530/324,315 536/23.51 435/69.4,240.2,252.3,252.33,320.1,325

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